| 1. | Study on serum - free medium for 5b1 cell growth
1细胞生长的无血清培养基的研究 |
| 2. | Methods : cell culture in serum - free medium , indirect immunofluorescence cytochemistry were used
方法：采用无血清细胞培养技术，间接免疫细胞化学染色法。 |
| 3. | We report a novel three - stage method to efficiently direct the differentiation of human embryonic stem cells into hepatic cells in serum - free medium
我们报道一种新的三步法有效诱导人胚胎干细胞在无血浆培养液中向肝细胞分化。 |
| 4. | The stable expression cell line was selected . expression level of the line cultured in serum - free medium was 580iu 106 cell 24h . the product expressed was purified by zn
筛选到的稳定表达株在无血清培养基的表达量为580iu 106细胞24h 。 |
| 5. | Materials and mehods nscs were isolated from e - 14 embryonic rat ' s brains , then cultured in serum - free medium with egf and bfgf ( 20ng / ml respectively ) as mitogens
12小时至36小时之间，死亡率上升最快: 36一48小时，死亡率几乎不再上升，稳定在60 %左右。 |
| 6. | However , the drawbacks of mammalian cell expression systems are apparent : they are difficult to culture in large scale , especially in serum - free medium ; the productivity is much lower than other expression systems and the costs are higher
但哺乳动物细胞表达系统的缺点也很明显，如表达量低、大规模培养困难、生产成本高昂等。 |
| 7. | Development of insect cell culture media and serum - free media , establishment of insect cell lines and applications of insect cell culture for production of biopesticides and expression of recombinant proteins in genetic engineering were reviewed in this paper
摘要综述了昆虫细胞培养基的现状和发展、无血清培养基的开发、昆虫细胞系的建立以及昆虫细胞在生物农药和重组蛋白中的应用。 |
| 8. | Results the epithelial cells cultured in serum - free medium not only demonstrated a significant increase in proliferative , colony - forming efficiency and cell generations , but also have the normal structural and functional properties than those in serum - containing medium
结果无血清培养系统中结膜上皮细胞的增殖、克隆形成及细胞传代能力均较含血清培养系统高，而且具备结膜上皮细胞全部组织结构及功能特点。 |
| 9. | Thus , the success of nscs therapy is determined by the induced differentiation and the effect of micro - enviroment on the survival , proliferation and differentiation of it . the isolation and culture of nscs in vitro successfully is the presupposition for its research . to avoid the influence of unknown factors exiting in serum on nscs " differentiation , serum - free medium with egf and bfgf as mitogens to stimulate proliferation and inhibit differentiation in vitro was introduced through a long period of search
淀粉样蛋白( amyloidpeptidy , a )是淀粉样蛋白前体蛋白( amyloidprecusorprotein ， app )异常代谢产生的含有39 ? 43个氨基酸残基的疏水肽，可聚合成不溶的纤维形式或不定型颗粒状结构，导致各种毒性反应，在阿尔茨海默病( alzheimer ' sdisease ， ad )发病中起着至关重要的作用。 |
| 10. | Subconfluent cultures of rat were maintained in dmem / 10 % fbs , 24hrs before neuronal induction , media were replaced with pre - induction media consisting of dmem / 10 % fbs / lmm beta - mercaptoethanol ( bme ) , to initiate neuronal differentiation , the preinduction media were removed , and the cells were washed with pbs and transferred to neuronal induction media composed of dmem / serum - free media , 5hrs later , 40ul dmso was given to every hole containing 2ml each
分别取第5代和第13代的mscs ，以8x10 cm ‘浓度接种于六孔板中的盖玻片上，制备细胞爬片，每孔加zinl培养液。达到80融合时，更换新鲜培养液，并在培养液中加人终浓度为lrnm的p一流基乙醇，诱导24小时， pbs洗涤，而后换成无血清的培养液。 |
